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KMID : 0613820120220121644
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Journal of Life Science
2012 Volume.22 No. 12 p.1644 ~ p.1650
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Polymorphisms and Allele Distribution of Novel Indel Markers in Jeju Black Cattle, Hanwoo and Imported Cattle Breeds
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Han Sang-Hyun
Cho In-Cheol
Kim Jae-Hwan
Cho Sang-Rae
Cho Won-Mo
Kim Sang-Geum
Kim Yoo-Kyung
Kang Yong-Jun
Park Yong-Sang
Kim Young-Hoon
Park Se-Phil
Kim Eun-Young
Lee Sung-Soo
Ko Moon-Suck
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Abstract
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The aim of this study was to screen the polymorphisms and distribution of each genotype of insertion/ deletion (indel) markers which were found in a preliminary comparative study of bovine genomic sequence databases. Comparative bioinformatic analyses were first performed between the nucleotide sequences of Bovine Genome Project and those of expressed sequence tag (EST) database, and a total of fifty-one species of indel markers were screened. Of these, forty-two indel markers were evaluated, and nine informative indel markers were ultimately selected for population analysis. Nucleotide sequences of each marker were re-sequenced and their polymorphic patterns were typed in six cattle breeds: Holstein, Angus, Charolais, Hereford, and two Korean native cattle breeds (Hanwoo and Jeju Black cattle). Cattle breeds tested in this study showed polymorphic patterns in eight indel markers but not in the Indel-15 marker in Charolais and Holstein. The results of analysis for Jeju Black cattle (JBC) population indicated an observed heterozygosity (Ho) that was highest in HW_G1 (0.600) and the lowest in Indel_29 (0.274). The PIC value was the highest in HW_G4 (0.373) and lowest in Indel_6 (0.305). These polymorphic indel markers will be useful in supplying genetic information for parentage tests and traceability and to develop a molecular breeding system for improvement of animal production in cattle breeds as well as in the JBC population.
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KEYWORD
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Indel marker, polymorphism, genotype, cattle, Jeju black cattle
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